Annuaire
Séminaire externe : Julien Béthune
| Quand ? |
Le 16/09/2024, de 11:00 à 12:00 |
|---|---|
| S'adresser à | Nawal Hajj Sleiman |
| Ajouter un événement au calendrier |
vCal
iCal
|
Séminaire externe : Julien Béthune (Hamburg University)
"Optimization and use of enzymes for proximity-dependent biotinylation”
Résumé :
Proximity-dependent biotinylation (PDB) has become a standard technique in the toolbox of methods available for the identification of protein-protein interactions. The most commonly used approach is based on the original BioID technique in which an abortive variant of a prokaryotic biotin protein ligase is fused to a protein of interest to mediate the non-specific biotinylation of vicinal proteins in living cells. While the first generation of PDB enzymes led to successful identification of protein-protein interactions, they also came with drawbacks, mostly bulky size and/or slow labeling kinetics. This triggered efforts to develop novel enzymes with improved properties to render the assay more versatile. Recently we engineered ultraID the smallest size and most efficient enzyme for PDB described to date. In this talk I will showcase how the enzyme can be used for probing otherwise difficult to access protein complexes but also for in vitro applications.
Hôte : Nawal Hajj Sleiman
Lundi 16 septembre
Salle 63