Directory
Bioscketch
Jonathan Enriquez, Ph.D.
Group Leader – Development and Function of Neuromuscular System
+33.7.68.66.90.89
lab website: https://lc.cx/pC8a
PERSONAL INFORMATION
ORCID: 0000-0002-7679-4674
Research ID: M-6945-2015
Date of birth: 15 March 1980
Nationality: French
URLs for websites: WEB page, facebook page, Twitter page
MAIN SCIENTIFIC CONTRIBUTIONS
My research integrates various fields, including genetics, molecular biology, imaging, post-imaging processing, computational biology, and behavioral approaches. Recently, I have also ventured into single cell and spatial transcriptomics. Using Drosophila melanogaster as my model organism, I aim to uncover novel biological concepts and developmental mechanisms.
Throughout the past decade, my expertise has focused on the development of three key components of the locomotor system: muscles, glia, and motoneurons. Within larval muscles, I have made significant discoveries regarding the transcriptional machinery responsible for determining their morphological identity (Enriquez et al., 2010, 2012, and 2015). Additionally, I pioneered the revelation that motoneurons exhibit a unique transcription factor code at the single cell level, which governs the morphology of their dendritic arbor and axonal targeting (Enriquez et al., 2015). To achieve this breakthrough, I developed the innovative MARCMbow technique, enabling the visualization of motoneurons at the single cell level while simultaneously modifying their genome.
As a group leader, I expanded my research to include the study of glial cells within the locomotor system. By introducing the cisMARCM technique (Enriquez et al., 2018), I demonstrated that glial cell development is not predetermined but rather exhibits plasticity and stochasticity. Although this work is sometimes viewed as a postdoc publication, I carried it out at Columbia University, where I was promoted to Associate Research Scientist and secured my first PI grant from the American ALS Association. With this funding, I initiated investigations into glial cells, which I continued at the IGFL with the support of my new research group.
My contributions as a co-corresponding author on a publication have gained recognition, particularly through its highlight in the 2018 F1000Prime Recommendation. Furthermore, recent studies have confirmed the conservation of glial cell development logic throughout evolution (Solène Clavreul et al., 2019).
In more recent endeavors, I utilized genetic manipulations and an innovative computational tool to reveal that transcription factor codes governing motoneuron morphologies are progressively established in immature motoneurons based on their birth order. Furthermore, we made a significant discovery indicating that RNA binding proteins post-transcriptionally regulate the establishment of these transcription factor codes in immature motoneurons, ultimately influencing the establishment of the axon muscle-connectome and the production of a precise number of MNs (Guan et al., 2022, 2023).
POSITIONS AND EMPLOYMENT
Since 2024 CNRS DR2 Second Grade Director of research, Institute of Functional Genomics of Lyon (IGFL), Ecole Normal Supérieur de Lyon, France
PREVIOUS POSITIONS
2015-2016 Associate Research Scientist
Department of Biochemistry & Molecular Biophysics, Columbia University, New York, USA
Building a functional locomotor system in Drosophila
2010-2015 Post-Doctoral Research Scientist
Mann Lab, Department of Biochemistry & Molecular Biophysics, Columbia University
Transcriptional identity code of adult motoneuron in Drosophila
2005 Undergraduate Internship (European Master of Genetics, 8 months)
Ecole Normale Supérieure of Paris, France
Role of the interaction centrosome-cortex in the regulation of cell polarity in Drosophila.
Mentor: François Schweisguth, Ph.D.
2004 Undergraduate Internship (European Master of Genetics, 6 months)
Steller Lab, Rockefeller University, New York, USA
Characterization of genes implicated in apoptosis in Drosophila.
Mentor: Bertrand Mollereau, Ph.D.
2003 Undergraduate Internship (European License of Genetics, 3 months)
Institute J. Monod, Paris, France
Study of apoptosis inhibition by Chlamydia pneumoniae in epithelial cells.
Mentor: David Ojcius, Ph.D.
2002 Undergraduate Internship (DEUG, 1 months)
Lyonnaise des Eaux/Suez Environnement, Bordeaux, France
Study of Densadeg and research of the optimal concentration of iron chloride for flocculation.
EDUCATION
2020 HDR: Habilitation à diriger les Recherches.
2010 PhD, Center of Developmental Biology, University of Toulouse 3, France
"Specification of muscle identity in Drosophila: The role of the Hox, Col/EBFand D- MyoD transcription factors"
PhD Supervisor: Alain Vincent, PhD.
2005 European Master of Genetics, University of Jussieu-Paris 7, France.
2005 Magistère of Genetics (selective school in France which awards the diploma based on a competitive evaluation process), University of Jussieu-Paris 7, France.
GRANTS
Total funding obtained through 8 Grants as Principal Investigator: 5304 K€.
2021/2023 Labex Grant (CORTEX). Collaborative project with Jean-Louis Bessereau team.
Role: Co-Principal Investigator (70 K€ per team)
2021/2029 Equipex+ Spatial-Cell-ID. Grant to set-up a spatial transcriptomic platform in Lyon.
Role: Co-Principal Investigator (4200 K€)
2021/2024 ANR JCJC grant
Role: Principal Investigator: (294 499€).
2018/2020 Elan ERC: awarded by the Idex program of Lyon University to selected scientist with potential to obtain ERC grants.
Role: Principal Investigator (42 000€)
2018-2020 Winner of the highly competitive Atip-Avenir fellowship co-sponsored with AFM Association
Role: Principal Investigator (360 000€)
2017-2020 ANR JCJC (286 783€). Note: declined as it overlapped with ATIP Avenir grant
2017-2019 Foundation of Medical Research grant (‘amorçage jeunes équipes’).
Role: Principal Investigator (296 600€)
2015-2016 Starter Grant, Amyotrophic Lateral Sclerosis Association, USA
Role: Principal Investigator (40,000$)
INSTITUTIONAL RESPONSIBILITIES
2021-2029 Member of the executive comity with two colleagues of the Equipex+ spatial-Cell-ID. The first national platform of spatial transcriptomic.
ORGANISATION OF SCIENTIFIC MEETINGS
2023 Member of the organizing committee for the 27th European Drosophila Research Conference (EDRC) (website), Lyon 2023. Expected number of participants: 700
2020 Member of the scientific committee for the 2nd MuSkLE conference, Saint-Etienne 2020. Expected number of participants: 120 (cancelled due to COVID19)
2019 Organizer of the 33rd Annual French Drosophila Conference, Lyon 2019. Number of participants: 90
2009 Assisted PhD adviser, Alain Vincent, in organising the Developmental French Society Meeting in Toulouse, France. Number of participants: 200
PUBLICATIONS
- Guan, W., Nie, Z., Laurençon, A., Bouchet, M., Godin, C., Kabir, C., Darnas, A., and Enriquez, J. (2023). The role of Imp and Syp RBPs in precise neuronal elimination by apoptosis through the regulation of TFs. eLife 12. 10.7554/eLife.91634.
- Bouchet M., Urdy S., Guan W., Kabir C., Garvis S., Enriquez J. A simple smiFISH pipeline to quantify mRNA at the single-cell level in 3D. (2023). STAR Protocols. Volume 4, Issue 2, 2023, 102316.
- Guan, W., Bellemin, S., Bouchet, M., Venkatasubramanian, L., Guillermin, C., Laurençon, A., Kabir, C., Darnas, A., Godin, C., Urdy, S., Mann, R.S., and Enriquez, J. (2022). Post-transcriptional regulation of transcription factor codes in immature neurons drives neuronal diversity. Cell Rep. 39.
- Babski, H., Jovanic, T., Surel. C., Yoshikawa, S., Zwart, MF., Valmier, J., Thomas, JB., Enriquez, J., Carroll P, Garcès, A. (2019) A GABAergic Maf-expressing interneuron subset regulates the speed of locomotion in Drosophila. Nat Commun. 2019 Oct 22;10(1):4796.
- Guan, W., Venkatasubramanian, L., Baek, M., Mann, R.S., and Enriquez, J. (2018). Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle. corresponding author. J. Vis. Exp. JoVE.
- Enriquez, J., Rio, L.Q., Blazeski, R., Bellemin, S., Godement, P., Mason, C., and Mann, R.S. (2018). Differing Strategies Despite Shared Lineages of Motor Neurons and Glia to Achieve Robust Development of an Adult Neuropil in Drosophila. Co- corresponding author. Neuron 97, 538–554.e5. Cover article: https://www.cell.com/neuron/issue?pii=S0896-6273(17)X0004-4#fullCover
- Hiesinger P: F1000Prime Recommendation 07 Aug 2018; 10.3410/f.732571328.793548920
- CNRS press: https://insb.cnrs.fr/fr/cnrsinfo/construire-un-systeme-nerveux-fonctionnel
- Enriquez, J., Venkatasubramanian, L., Baek, M., Peterson, M., Aghayeva, U., and Mann, R.S. (2015). Specification of individual adult motor neuron morphologies by combinatorial transcription factor codes. Co- corresponding author. Neuron 86, 955–970.
- Drosophila image award: http://drosophila-images.org/2016
- Highlight: Tuthill, J. (2015). A framework for fatalism in the fly. J. Exp. Biol. 218, 3349–3350.
- Commented in a chapter book: Hobert, O. (2016). Chapter Twenty-Five - Terminal Selectors of Neuronal Identity. In Current Topics in Developmental Biology, P.M. Wassarman, ed. (Academic Press), pp. 455–475.
- Baek, M., Enriquez, J., and Mann, R.S. (2013). Dual role for Hox genes and Hox co-factors in conferring leg motoneuron survival and identity in Drosophila. Dev. Camb. Engl. 140, 2027–2038.
- Boukhatmi, H., Frendo, J.L., Enriquez, J., Crozatier, M., Dubois, L., and Vincent, A. (2012). Tup/Islet1 integrates time and position to specify muscle identity in Drosophila. Dev. Camb. Engl. 139, 3572–3582.
- Enriquez, J., de Taffin, M., Crozatier, M., Vincent, A., and Dubois, L. (2012). Combinatorial coding of Drosophila muscle shape by Collier and Nautilus. Dev. Biol. 363, 27–39.
- Enriquez, J. and Vincent, A. (2010). Segmental variations in the patterns of somatic muscles: what roles for Hox? Fly (Austin) 4, 249–252
- Enriquez, J., Boukhatmi, H., Dubois, L., Philippakis, A.A., Bulyk, M.L., Michelson, A.M., Crozatier, M., and Vincent, A. (2010). Multi-step control of muscle diversity by Hox proteins in the Drosophila embryo. Dev. Camb. Engl. 137, 457–466.
- Dubois, L., Enriquez, J., Daburon, V., Crozet, F., Lebreton, G., Crozatier, M., and Vincent, A. (2007). Collier transcription in a single Drosophila muscle lineage: the combinatorial control of muscle identity. Dev. Camb. Engl. 134, 4347–4355.