Jonathan Enriquez, Ph.D.
Group Leader – Development and Function of Neuromuscular System
lab website: https://lc.cx/pC8a
Research ID: M-6945-2015
Date of birth: 15 March 1980
MAIN SCIENTIFIC CONTRIBUTIONS
My research integrates various fields, including genetics, molecular biology, imaging, post-imaging processing, computational biology, and behavioral approaches. Recently, I have also ventured into single cell and spatial transcriptomics. Using Drosophila melanogaster as my model organism, I aim to uncover novel biological concepts and developmental mechanisms.
Throughout the past decade, my expertise has focused on the development of three key components of the locomotor system: muscles, glia, and motoneurons. Within larval muscles, I have made significant discoveries regarding the transcriptional machinery responsible for determining their morphological identity (Enriquez et al., 2010, 2012, and 2015). Additionally, I pioneered the revelation that motoneurons exhibit a unique transcription factor code at the single cell level, which governs the morphology of their dendritic arbor and axonal targeting (Enriquez et al., 2015). To achieve this breakthrough, I developed the innovative MARCMbow technique, enabling the visualization of motoneurons at the single cell level while simultaneously modifying their genome.
As a group leader, I expanded my research to include the study of glial cells within the locomotor system. By introducing the cisMARCM technique (Enriquez et al., 2018), I demonstrated that glial cell development is not predetermined but rather exhibits plasticity and stochasticity. Although this work is sometimes viewed as a postdoc publication, I carried it out at Columbia University, where I was promoted to Associate Research Scientist and secured my first PI grant from the American ALS Association. With this funding, I initiated investigations into glial cells, which I continued at the IGFL with the support of my new research group.
My contributions as a co-corresponding author on a publication have gained recognition, particularly through its highlight in the 2018 F1000Prime Recommendation. Furthermore, recent studies have confirmed the conservation of glial cell development logic throughout evolution (Solène Clavreul et al., 2019).
In more recent endeavors, I utilized genetic manipulations and an innovative computational tool to reveal that transcription factor codes governing motoneuron morphologies are progressively established in immature motoneurons based on their birth order. Furthermore, we made a significant discovery indicating that RNA binding proteins post-transcriptionally regulate the establishment of these transcription factor codes in immature motoneurons, ultimately influencing the establishment of the axon muscle-connectome and the production of a precise number of MNs (Guan et al., 2022, 2023).
POSITIONS AND EMPLOYMENT
2018-2019 Facility manager of the arthro-tools facility at ENS Lyon. The facility develops innovative research projects based on arthropod models for 10 teams and organize monthly meetings.
2015-2016 Associate Research Scientist
Department of Biochemistry & Molecular Biophysics, Columbia University, New York, USA
Building a functional locomotor system in Drosophila
2010-2015 Post-Doctoral Research Scientist
Mann Lab, Department of Biochemistry & Molecular Biophysics, Columbia University
Transcriptional identity code of adult motoneuron in Drosophila
2005 Undergraduate Internship (European Master of Genetics, 8 months)
Ecole Normale Supérieure of Paris, France
Role of the interaction centrosome-cortex in the regulation of cell polarity in Drosophila.
Mentor: François Schweisguth, Ph.D.
2004 Undergraduate Internship (European Master of Genetics, 6 months)
Steller Lab, Rockefeller University, New York, USA
Characterization of genes implicated in apoptosis in Drosophila.
Mentor: Bertrand Mollereau, Ph.D.
2003 Undergraduate Internship (European License of Genetics, 3 months)
Institute J. Monod, Paris, France
Study of apoptosis inhibition by Chlamydia pneumoniae in epithelial cells.
Mentor: David Ojcius, Ph.D.
2002 Undergraduate Internship (DEUG, 1 months)
Lyonnaise des Eaux/Suez Environnement, Bordeaux, France
Study of Densadeg and research of the optimal concentration of iron chloride for flocculation.
2020 HDR: Habilitation à diriger les Recherches.
2010 PhD, Center of Developmental Biology, University of Toulouse 3, France
"Specification of muscle identity in Drosophila: The role of the Hox, Col/EBFand D- MyoD transcription factors"
PhD Supervisor: Alain Vincent, PhD.
2005 European Master of Genetics, University of Jussieu-Paris 7, France.
2005 Magistère of Genetics (selective school in France which awards the diploma based on a competitive evaluation process), University of Jussieu-Paris 7, France.
2023 Invited lecturer, the genetics of Drosophila, East China Normal University, China, 10 hours
Invited lecturer, myogenesis in vertebrates and invertebrates, University of Lyon I, France, M2 students, 2 hours
2022 Invited lecturer, building the connectome, M2 students UCBL, Lyon 2 hours
Invited lecturer, building the axon-muscle connectome and spatial transcriptomic techniques, L3 students, ENS Lyon, 4 hours
2022-2023 Co-organizer of the graduate school Muskle (website), Lyon, one week (I did one full day of practical work, and a conference of 1 hour) .
2021 Invited lecturer, building the axon-muscle connectome, ENS Lyon, L3 students, 4 hours
Invited lecturer, the genetic tool kit of Drosophila, East China Normal University, China, M2 students, 4 hours
2020 Member of the evaluation committee to evaluate oral presentations of Master 2 students
2019 Invited lecturer, myogenesis in vertebrate and invertebrate courses for graduate students, ENS of Lyon, France, 4 hours
Member of the evaluation committee to evaluate oral presentations of Master 2 students
2018 Invited lecturer, development of Drosophila melanogaster courses for undergraduate students, ENS of Lyon, France, 15 hours
2006-2008 Graduate Teaching Assistant, developmental biology courses for graduates and undergraduate students, University of Toulouse 3, France, 64 hours/year
Total number (6): 2 postdoctoral, 2 doctoral, 2 predoctoral
2013-2015 Post-Doctoral fellowship, French Association against Muscular Dystrophy, France
2010-2012 Philippe Foundation Fellowship, USA
2008-2009 Doctoral fellowship, AFM (French Muscular Dystrophy Association), France
2005-2008 Doctoral fellowship, allocated to the best Master students in France
2004-2005 Pre-doctoral fellowship, French Ministry for Research and Technology, France
2004 Undergraduate Fellowship, French State internship at the Rockefeller University, USA
HONORS AND AWARDS
2019 Camille Guillemin, my PhD student, obtained the highly competitive master prize of the French society of Myology in November 2018. The selection was based on her CV, project and scientific quality of the supervisor. Prize: 1000 euros for her and 19000 euros for the laboratory.
2016 Finalist of Drosophila Image Award https://drosophila-images.org/2016-2/
Total funding obtained through 8 Grants as Principal Investigator: 5304 K€.
2021/2023 Labex Grant (CORTEX). Collaborative project with Jean-Louis Bessereau team.
Role: Co-Principal Investigator (70 K€ per team)
2021/2029 Equipex+ Spatial-Cell-ID. Grant to set-up a spatial transcriptomic platform in Lyon.
Role: Co-Principal Investigator (4200 K€)
2021/2024 ANR JCJC grant
Role: Principal Investigator: (294 499€).
2018/2020 Elan ERC: awarded by the Idex program of Lyon University to selected scientist with potential to obtain ERC grants.
Role: Principal Investigator (42 000€)
2018-2020 Winner of the highly competitive Atip-Avenir fellowship co-sponsored with AFM Association
Role: Principal Investigator (360 000€)
2017-2020 ANR JCJC (286 783€). Note: declined as it overlapped with ATIP Avenir grant
2017-2019 Foundation of Medical Research grant (‘amorçage jeunes équipes’).
Role: Principal Investigator (296 600€)
2015-2016 Starter Grant, Amyotrophic Lateral Sclerosis Association, USA
Role: Principal Investigator (40,000$)
SUPERVISION OF GRADUATE STUDENTS AND POSTDOCTORAL FELLOWS
Current supervision in the lab: 1 Master Students, 3 PhD.
Camille Guillemin, my first PhD student, obtained the highly competitive master prize of the French society of Myology in November 2018. The selection was based on her CV, project and scientific quality of the supervisor. Prize: 1000 euros for her and 19000 euros for the laboratory.
Past supervision in the lab: 8 Master Students, 2 postdoc.
Co-supervised: 3 PhDs, 2 Master Students and 1 Technician in Mann lab, Columbia University, New York, USA. 2 PhDs in Vincent lab, University of Toulouse 3, France.
2021-2029 Member of the executive comity with two colleagues of the Equipex+ spatial-Cell-ID. The first national platform of spatial transcriptomic.
2022-2024 Graduate Student advisor of 3 students, Lyon University and Toulouse III, France.
2023 Thesis Jury Examiner of 1 PhD, in Clermont Auvergne University, 1 in
Marseille University, France
2022 Thesis Jury Examiner of 2 PhD, Toulouse, University Paul Savatier and Lyon, Université Claude Bernard
2021-2023 Member of the evaluating comity of the ‘l'Ecole de l'Inserm Liliane Bettencourt’, which give around 20 fellowship to MD student to do a PHD
2020-2022 Mission head for the IGFL direction at the UAR Biosciences (UAR3444/US8). Platform
regrouping several facilities
2019-2022 Graduate Student advisor of 2 students, Lyon University, France.
2017-2020 Graduate Student advisor of 2 students, ENS Lyon, France.
2018-2019 Thesis Jury Examiner of 3 PhD, 2 in Clermont Auvergne University, 1 in
Montpellier University, France
2018 Member of the selection of committee for an assistant professor, Lyon University.
2018-2019 Facility manager of the arthro-tools facility (website) at ENS Lyon. The facility develops innovative research projects based on arthropod models for 10 teams and organizes monthly meeting.
MEMBERSHIPS OF SCIENTIFIC SOCIETIES
Member of the French Society of Myology (since 2019);
Member of the French Society of developmental Biology (since 2017);
Member of the Magistère of genetic association (since 2016).
INVITED SPEAKER AT INTERNATIONAL INSTITUTES
MeLisS institute, France, ‘Building the axon-muscle connectome’ 2023
Drosophila development and genetics in Toulouse, 35 years and beyond, France. ‘Shaping the axon-muscle connectome’. 2021
Multiscale imaging of Life Lyon, Lyon, France. The post-transcriptional regulation of TFs in immature motoneurons shapes the axon-muscle connectome. 2021
Club Neuromusculaire Auvergne-Rhône/Alpes, France, ‘Building the correct innervation’, 2019
Center of Developmental Biologie, Toulouse, France, ‘Motoneurons and Glia: Common origins yet divergent modes of development’, 2019
Institut de Myologie, Paris, France, ‘Making unique connections’, 2018
Institut Valrose de Nice, France, ‘Motoneurons and glia: Common origins yet divergent modes of development’, 2018
The Munich Center for Neurosciences, Germany, ‘Motoneurons and Glia: Common origins yet divergent modes of development’, 2017
Institute for Research in Biomedicine (IRB), Barcelona, Spain, ‘Motoneurons and Glia: Common origins yet divergent modes of development’, 2017
Club Neuromusculaire Auvergne-Rhône/Alpes, Lyon, France, ‘Development of the locomotor system’ 2017
The Fly Eye Club Meeting at Rockefeller University, New York, USA, ‘Making a motoneuron unique’, 2016
Memorial Sloan Ketering Cancer Center, USA, ‘Making a motoneuron unique’, 2014
ORAL COMMUNICATIONS AT INTERNATIONAL MEETINGS
Joint meeting of the Portuguese, Spanish and French societies for Developmental Biologie, Porto, Portugal, November 2018
Jacques Monod Conference “Building, repairing and evolving biological tissues”, Roscoff, France, 2015
The 56th Annual Drosophila Research Conference, Chicago, USA, 2015
The Developmental French Society Meeting in Toulouse, France, 2009
NeuroFly, Saint Malo, France, September 2022
Modeling cell fate, Roscoff, France, September 2018
Gordon Research Conference on Neural Development, Newport, USA, 2016
The 56th Annual Drosophila Research Conference, Chicago, USA, 2015
The Neurobiology of Drosophila Meeting, Cold Spring Harbor, USA, 2013
The 8th FENS Forum of Neuroscience, Barcelona, Spain, 2012
‘Making Muscle in the Embryo and the Adult’ Meeting at Columbia University, New York, USA, 2009
ORGANISATION OF SCIENTIFIC MEETINGS
2023 Member of the organizing committee for the 27th European Drosophila Research Conference (EDRC) (website), Lyon 2023. Expected number of participants: 700
2020 Member of the scientific committee for the 2nd MuSkLE conference, Saint-Etienne 2020. Expected number of participants: 120 (cancelled due to COVID19)
2019 Organizer of the 33rd Annual French Drosophila Conference, Lyon 2019. Number of participants: 90
2009 Assisted PhD adviser, Alain Vincent, in organising the Developmental French Society Meeting in Toulouse, France. Number of participants: 200
Reviewer for Developmental Biology, JOVE, iScience, Embo reports, Neural Development journals
Review for funding agencies:
2020, Reviewer for the Research Grant awards from the Human Frontier Science Program Organization.
2018-2019, Reviewer of the French National French Agency (ANR)
2018-present, Scientific Expert member of the molecular and cellular neuroscience panel of ANR
- Guan, W., Nie, Z., Laurençon, A., Bouchet, M., Godin, C., Kabir, C., Darnas, A., and Enriquez, J. (2023). The role of Imp and Syp RBPs in precise neuronal elimination by apoptosis through the regulation of TFs. eLife 12. 10.7554/eLife.91634.
- Bouchet M., Urdy S., Guan W., Kabir C., Garvis S., Enriquez J. A simple smiFISH pipeline to quantify mRNA at the single-cell level in 3D. (2023). STAR Protocols. Volume 4, Issue 2, 2023, 102316.
- Guan, W., Bellemin, S., Bouchet, M., Venkatasubramanian, L., Guillermin, C., Laurençon, A., Kabir, C., Darnas, A., Godin, C., Urdy, S., Mann, R.S., and Enriquez, J. (2022). Post-transcriptional regulation of transcription factor codes in immature neurons drives neuronal diversity. Cell Rep. 39.
- Babski, H., Jovanic, T., Surel. C., Yoshikawa, S., Zwart, MF., Valmier, J., Thomas, JB., Enriquez, J., Carroll P, Garcès, A. (2019) A GABAergic Maf-expressing interneuron subset regulates the speed of locomotion in Drosophila. Nat Commun. 2019 Oct 22;10(1):4796.
- Guan, W., Venkatasubramanian, L., Baek, M., Mann, R.S., and Enriquez, J. (2018). Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle. corresponding author. J. Vis. Exp. JoVE.
- Enriquez, J., Rio, L.Q., Blazeski, R., Bellemin, S., Godement, P., Mason, C., and Mann, R.S. (2018). Differing Strategies Despite Shared Lineages of Motor Neurons and Glia to Achieve Robust Development of an Adult Neuropil in Drosophila. Co- corresponding author. Neuron 97, 538–554.e5. Cover article: https://www.cell.com/neuron/issue?pii=S0896-6273(17)X0004-4#fullCover
- Hiesinger P: F1000Prime Recommendation 07 Aug 2018; 10.3410/f.732571328.793548920
- CNRS press: https://insb.cnrs.fr/fr/cnrsinfo/construire-un-systeme-nerveux-fonctionnel
- Enriquez, J., Venkatasubramanian, L., Baek, M., Peterson, M., Aghayeva, U., and Mann, R.S. (2015). Specification of individual adult motor neuron morphologies by combinatorial transcription factor codes. Co- corresponding author. Neuron 86, 955–970.
- Drosophila image award: http://drosophila-images.org/2016
- Highlight: Tuthill, J. (2015). A framework for fatalism in the fly. J. Exp. Biol. 218, 3349–3350.
- Commented in a chapter book: Hobert, O. (2016). Chapter Twenty-Five - Terminal Selectors of Neuronal Identity. In Current Topics in Developmental Biology, P.M. Wassarman, ed. (Academic Press), pp. 455–475.
- Baek, M., Enriquez, J., and Mann, R.S. (2013). Dual role for Hox genes and Hox co-factors in conferring leg motoneuron survival and identity in Drosophila. Dev. Camb. Engl. 140, 2027–2038.
- Boukhatmi, H., Frendo, J.L., Enriquez, J., Crozatier, M., Dubois, L., and Vincent, A. (2012). Tup/Islet1 integrates time and position to specify muscle identity in Drosophila. Dev. Camb. Engl. 139, 3572–3582.
- Enriquez, J., de Taffin, M., Crozatier, M., Vincent, A., and Dubois, L. (2012). Combinatorial coding of Drosophila muscle shape by Collier and Nautilus. Dev. Biol. 363, 27–39.
- Enriquez, J. and Vincent, A. (2010). Segmental variations in the patterns of somatic muscles: what roles for Hox? Fly (Austin) 4, 249–252
- Enriquez, J., Boukhatmi, H., Dubois, L., Philippakis, A.A., Bulyk, M.L., Michelson, A.M., Crozatier, M., and Vincent, A. (2010). Multi-step control of muscle diversity by Hox proteins in the Drosophila embryo. Dev. Camb. Engl. 137, 457–466.
- Dubois, L., Enriquez, J., Daburon, V., Crozet, F., Lebreton, G., Crozatier, M., and Vincent, A. (2007). Collier transcription in a single Drosophila muscle lineage: the combinatorial control of muscle identity. Dev. Camb. Engl. 134, 4347–4355.